Morrisons has said it will shut 52 in-store cafes along with some of its convenience stores, florists, meat and fish counters and pharmacies.
The supermarket said the closures are part of a shake-up which will result in 365 people facing redundancy.
Eighteen market kitchens, 17 convenience stores, 13 florists, 35 meat counters, 35 fish counters and four pharmacies will also be affected.
The in-store cafe at Morrisons Gloucester on Metz Way is among the cafes that will shut as a result of the announcement.